Nucleic Acid Testing
Current template:PCR Kit Template
Quantitative Real-Time PCRHigh Sensitivity
PCR DETECTION KIT
PCR Test Kit for Detecting Maize-Derived Components
10 copies
Limit of Detection
40-45
Cyclic number
Citation Standards
GB/T 27405-2008National Food Safety Standard: Microbiological Examination of Foods — PCR Detection Method
SN/T 1193-2019Nucleic Acid Detection Methods for Foodborne Pathogens in Imported and Exported Foods: Real-Time Fluorescent PCR Method
ISO 22174:2005Microbiology of food and feed — PCR method for detecting genetically modified organisms
Technical Principles
Real-time PCR
Enzymatic reaction based on DNA polymerase that uses real-time fluorescence monitoring of PCR amplification to achieve qualitative or quantitative detection of target nucleic acids.
Nucleic Acid Extraction
Extract target DNA/RNA from samples and remove inhibitors such as proteins and polysaccharides to obtain high-purity nucleic acid templates for PCR.
PCR amplification
Under the guidance of specific primers and fluorescent probes, DNA polymerase catalyzes dNTP incorporation to synthesize a new strand, releasing one fluorescent signal per amplification cycle.
Fluorescence Detection
The instrument acquires fluorescence signals in real time and performs precise quantification of target nucleic acids by comparing Ct values against a standard curve.
Core Performance Metrics
Core Technical Specifications
Rigorous quality control system ensures stability and reliability for every product batch.
Limit of Detection (LOD)
Minimum 10 copies/reaction
Specificity
No cross-reactivity
Linear Range
10^1 - 10^8 copies/mL
Repeatability (CV)
≤3%
Reaction System
25 μL / 50 μL
Packaging Size
48T/box
Amplification Procedure
Standard Amplification Procedure
Optimized reaction conditions to achieve the best balance between amplification efficiency and specificity.
| Steps | Temperature | Time | Cyclic Number | Description |
|---|---|---|---|---|
| pre-deformation | 95°C | 3-5 min | 1 | Activate DNA polymerase |
| gender transition | 95°C | 10-15 sec | 40-45 | Collect fluorescence signal |
| Annealing/Extension | 55-60°C | 30-40 sec |
Note: Specific reaction conditions may vary depending on the instrument model and target analyte. Please refer to the product manual or consult a technical expert.
Compatible Instruments
Compatible Instruments
Widely compatible with mainstream real-time PCR instruments to meet diverse laboratory equipment needs
ABI 7500 / 7500 Fast
LightCycler 480 II
CFX96 / CFX384
QuantStudio 5 / 6 / 7
Mx3000P / Mx3500P
Rotor-Gene Q
Storage Conditions
Reagent Storage
Store below 20°C in the dark. Valid for 12 months.
Shipping Conditions
Low-temperature cold chain transport to prevent repeated freeze-thaw cycles.
Storage after reconstitution
2-8°C for stable storage of 7 days; long-term storage at -20°C
Important Notes
Not intended for clinical diagnosis.
Wear a lab coat and gloves during operation to prevent contamination.
Vortex all reagents thoroughly and briefly centrifuge prior to use.
Prepare the reaction mixture in a laminar flow hood or biosafety cabinet.
Product Details
Product Details
Downloads & Support
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